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Oxford Nanopore 1d native barcoding genomic dna kit expnbd104
1d Native Barcoding Genomic Dna Kit Expnbd104, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1d+native+barcoding+genomic+dna+kit/rapid+barcoding+kit+sqk+rbk004/pm39802675-144-15-38
Average 90 stars, based on 1 article reviews
1d native barcoding genomic dna kit expnbd104 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Sequencing:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation
Article Snippet: Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [ ]. .. MinION sequencing was carried out following the manufacturer’s instructions using the 1D Native barcoding genomic DNA Kit (Nanopore, EXP-NBD104 and SQK-LSK109, Oxford Nanopore-technology (ONT)) on a Spot-ON flow cell (R9.4.1; ONT). ..

Amplification:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Construct:

Article Title: Comparison of assembly platforms for the assembly of the nuclear genome of Trichoderma harzianum strain PAR3.
Article Snippet: For short-read sequencing, amplified genomic DNA was used to construct a 150 paired-end library using a HiSeq PE150 kit (Illumina, San Diego, CA, U.S.A.), and then sequenced on an Illumina HiSeq 2500 with 2 × 150 bp paired-end format. .. For long-read sequencing, amplified genomic DNA was used to construct a sequencing library using a 1D Native barcoding genomic DNA kit from Oxford Nanopore Technologies (ONT) (Alameda, CA, U.S.A.), and then this library was sequenced by an ONT minION system. .. Illumina sequencing produced 141,612,983 paired-end reads for a total of 104.68 Gb (Q > 30) and 518X predicted coverage.

Multiplexing:

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes.
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.

Ligation:

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes.
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.

Article Title: Functional alterations and predictive capacity of gut microbiome in type 2 diabetes
Article Snippet: DNA end repair was performed using the NEBNext FFPE Repair Mix (New England Biolabs (NEB), Evry, France). .. We used NEBNext Ultra II End Repair/dA-Tailing Module (NEB) for the “end prep” step, 1D Native barcoding genomic DNA kit (Oxford Nanopore Technologies (ONT)), and “NEB Blunt/TA Ligase Master Mix kit (NEB) for DNA multiplexing and adapters ligation. .. Agentcourt AMPure XP (Beckman Coulter, Villepinte, France) beads were used for DNA purification.



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